CHROMOSOME WALKING Meaning and
Definition
-
Chromosome walking is a molecular biology technique used to isolate and sequence specific genes or other DNA sequences within a DNA molecule or chromosome. It involves the systematic identification and isolation of neighboring DNA segments or genes, in order to "walk" along the chromosome and study multiple genes or segments of DNA.
In chromosome walking, a known DNA fragment or gene is used as a probe to identify and clone neighboring DNA fragments. This can be accomplished by creating a DNA library of large fragments of chromosomal DNA and subjecting it to a series of hybridization experiments with the probe. The hybridized fragments are then isolated, amplified, and used as probes for the subsequent rounds of hybridization. This iterative process allows the determination of the order and arrangement of the genes or DNA segments along the chromosome, effectively "walking" along the chromosome.
Chromosome walking serves as an effective tool to study the physical arrangement of genes, identify neighboring genes, and investigate gene organization, regulation, and function. It has been widely used in various research fields including genetics, genomics, and molecular biology. Chromosome walking has contributed significantly to the mapping of genomes, the identification of disease-causing genes, and the understanding of the molecular basis of various genetic disorders.
Common Misspellings for CHROMOSOME WALKING
- xhromosome walking
- vhromosome walking
- fhromosome walking
- dhromosome walking
- cgromosome walking
- cbromosome walking
- cnromosome walking
- cjromosome walking
- curomosome walking
- cyromosome walking
- cheomosome walking
- chdomosome walking
- chfomosome walking
- chtomosome walking
- ch5omosome walking
- ch4omosome walking
- chrimosome walking
- chrkmosome walking
- chrlmosome walking
- chrpmosome walking
Etymology of CHROMOSOME WALKING
The term "chromosome walking" was coined by geneticists and molecular biologists to describe a technique used to locate and sequence specific DNA regions on a chromosome. The word "chromosome" refers to the thread-like structures found in the nucleus of cells that contain genetic information, while "walking" implies the progressive movement through the chromosome.
The term was introduced in the early 1980s when DNA sequencing methods were being developed. Chromosome walking involves using a known DNA sequence as a starting point to identify adjacent regions on the same chromosome. By "walking" along the chromosome, scientists can progressively sequence and map larger regions of DNA.
The choice of the word "walking" reflects the step-by-step approach used in this technique, moving forward in a methodical manner, much like taking steps while walking.
Infographic
Add the infographic to your website:
